Highly efficient in vitro photodynamic inactivation of Mycobacterium smegmatis
نویسندگان
چکیده
منابع مشابه
Highly efficient in vitro photodynamic inactivation of Mycobacterium smegmatis.
OBJECTIVES Efforts to control tuberculosis (TB) have been hampered by the emergence of multiple-drug resistant strains, necessitating pursuit of alternative approaches to the current antibiotic-based treatments. Herein, we explore the feasibility of photodynamic inactivation (PDI) of mycobacteria. METHODS In vitro PDI studies employing Mycobacterium smegmatis as a surrogate for Mycobacterium ...
متن کاملMICROBIAL TRANSFORMATION OF CHOLESTEROL BY MYCOBACTERIUM SMEGMATIS
Mycobacterium smegmatis PTCC 1307 (CIP 73.26) was used as a microbial agent to produce androsta-1,4-diene-3,17-dione (ADD) and androst-4-ene-3,17-dione, two useful precursors in the synthesis of steroid drugs. The side chain of cholesterol, as the substrate, was selectively cleaved in the presence of five enzyme inhibitors. An intermediate structure with intact side chain, cholest-4-ene-3-one, ...
متن کاملA Kinetic Study of In Vitro Lysis of Mycobacterium smegmatis.
The traditional diagnostic tests for tuberculosis consist of an acid fast stain and a culture test from a sputum sample. With the emergence of drug resistant strains of tuberculosis, nucleic acid amplification has become the diagnostic test of choice. The nucleic acid amplification test consists of four steps: sputum sample collection, lysis of bacilli to release DNA, DNA amplification by PCR a...
متن کاملPhotodynamic inactivation of in vitro bacterial cultures from pressure ulcers.
PURPOSE To evaluate in vitro the antibacterial effect of diode laser light of wavelength 650 nm, in association with the photosensitive substance toluidine blue, on the bacteria in infected skin ulcers. METHODS Samples were collected by means of swabs containing a medium for transporting infected material from skin ulcers. The material was inoculated into culturing medium containing azide blo...
متن کاملPhotodynamic Inactivation of Endopathogenic Microbiota Using Curcumin- mediated Antimicrobial Photodynamic Therapy
Root canal disinfection is one of the main factors governing success of endodontic therapy. Antimicrobial photodynamic therapy (aPDT) is presented as a promising antimicrobial therapy that can eliminate microbiota present in infected root canal systems. In this study, a series of experiments investigated the effects of aPDT on cell viability and biofilm degradation ability of endopathogenic mic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2009
ISSN: 1460-2091,0305-7453
DOI: 10.1093/jac/dkp278